Many renowned nattuvanars and dancers of that period taught at the institute. Among them were Meenakshisundaram Pillai, Rukmini Devi's first teacher, Muthukumara Pillai, and Chokkalingam Pillai. Karaikkal Saradambal Ammal, known for her nritta, polished the technique of the early students. Dandayudapani Pillai later joined the staff, as did Mylapore Gowri Ammal. These early teachers bequeathed many compositions and stylistic inputs to the institute which remain embedded in the Kalakshetra style today. Ambu Pannikar, the great Kathakali ashaan who spent the last six years of his life at Kalakshetra, taught Rukmini Devi several Kathakali movements and set pieces that were used to great effect in her dance dramas. After his death, another doyen - Chandu Pannikar came to the college, bringing along with him young boys, Dhananjayan, Balagopalan, and later, his own son Janardhanan. These three, along with the older Kunhiraman, Ambu Pannikar's son, became the early male dancers of the institute, participating in the new dramas that Rukmini Devi choreographed. They became known
for their heroic roles in Kalakshetra's dance dramas.
Rukmini Devi personally trained the early dance students, who then took on the responsibility of teaching new students. She held a special morning class where she taught ballet exercises.